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Rabbit抗原名称
Sp7/Osterix分子别名
Transcription factor Sp7; Zinc finger protein osterix; OSX免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q8TDD2克隆号
SDT-3238-16抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Saos-2, mouse articular cartilage, mouse embryo纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu IF 1:1000 Ms, Rt
Sp7/Osterix is a ~45 kDa zinc-finger transcription factor of the Sp/KLF family that is indispensable for osteoblast lineage commitment and bone formation, acting downstream of Runx2 and cooperatively with BMP-6 to drive expression of osteogenic genes such as COL1A1, ALP, OCN, SATB2 and SOST, while its own transcription is induced by BMP-2, IGF-I and PTH and its activity is tuned by Akt- and p38 MAPK-mediated phosphorylation, ubiquitin–proteasome degradation and autoregulation via an OSX-bound tandem repeat in its promoter, and genetic or regulatory disruption of this short-lived nuclear protein underlies skeletal disorders including osteogenesis imperfecta and osteoporosis.
免疫印迹
WB result of Sp7/Osterix Recombinant Rabbit mAb
Primary antibody: Sp7/Osterix Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Saos-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 45, 50 kDaWB result of Sp7/Osterix Recombinant Rabbit mAb
Primary antibody: Sp7/Osterix Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse articular cartilage lysate 20 µg
Lane 3: mouse embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 45, 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human chondroma. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Saos2 cells. Anti- Sp7/Osterix antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse E14 embryo. Anti- Sp7/Osterix antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat E14 embryo. Anti- Sp7/Osterix antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Sp7/Osterix in tumor tissue.
Expression of Sp7/Osterix in human tissue.
Expression of Sp7/Osterix in mouse & rat tissue.
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