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Rabbit抗原名称
SLC6A1分子别名
Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1; GABATR; GABT1; GAT1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P30531克隆号
SDT-2424-278抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu, Ms, Rt IF 1:500 Rt
SLC6A1 encodes GAT-1, the principal Na⁺-coupled transporter that clears the inhibitory neurotransmitter GABA from the synaptic cleft by pumping it back into neurons and astrocytes, thereby terminating GABAergic signaling and maintaining excitatory–inhibitory balance throughout the CNS; mutations that misfold GAT-1 or abolish its plasma-membrane trafficking reduce synaptic GABA clearance, causing epilepsy, intellectual disability, autism spectrum disorders, ADHD, and mood dysfunction, whereas chemical or pharmacological chaperones can rescue some variants by restoring correct folding and surface expression.
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded rat retina. Anti-SLC6A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of SLC6A1 in tumor tissue.
Expression of SLC6A1 in tumor tissue.
Expression of SLC6A1 in mouse & rat tissue.
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