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宿主来源
Rabbit抗原名称
SEZ6分子别名
Seizure protein 6 homolog; SEZ-6; hSEZ-6免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q53EL9克隆号
SDT-2097-7抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
H69纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu IHC-P 1:250 Hu
SEZ6 (Seizure-related gene 6) is a type I transmembrane glycoprotein (~110 kDa) composed of five short consensus repeat (SCR) domains, three CUB domains, a single transmembrane segment and a short cytoplasmic tail that is predominantly expressed in the central nervous system, where it acts as a critical trafficking chaperone for kainate receptor subunits GluK2/3, ensuring their proper N-glycosylation (including the HNK-1 epitope), post-ER export and surface localization to regulate synaptic connectivity, long-term potentiation and dendritic arbor balance, and is sequentially cleaved by BACE1 and γ-secretase to release a soluble ectodomain whose CSF level serves as a direct pharmacodynamic read-out of BACE1 inhibition, while in neuroendocrine neoplasms its selective over-expression is exploited as an ADC target for therapies against small-cell lung cancer and related tumors.
免疫印迹
WB result of SEZ6 Recombinant Rabbit mAb
Primary antibody: SEZ6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: H69 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 130-200 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SEZ6 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-SEZ6 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SEZ6 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded H69 cells. Anti-SEZ6 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded HeLa cells. Anti-SEZ6 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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