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宿主来源
Rabbit抗原名称
CK1α分子别名
Casein kinase I isoform alpha; CKI-alpha; CK1; CSNK1A1免疫原
Synthetic Peptide细胞定位
Nucleus, Cytoplasm, CytoskeletonAccession
P48729克隆号
SDT-2884-60抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
PC-3, Caco-2, MCF7, HeLa, NIH/3T3, mouse spleen, rat brain, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:100 Hu
CK1α (casein kinase 1 isoform alpha) is a ubiquitously expressed, constitutively active serine/threonine kinase that docks to a conserved recognition motif (pSer/pThr-X-X-S/T) in hundreds of cytoplasmic, nuclear and membrane substrates, thereby governing Wnt/β-catenin signaling by phosphorylating β-catenin for β-TrCP-mediated degradation, synchronizing circadian rhythms through PER protein turnover, regulating DNA-damage checkpoints by modifying p53 and MDM2, driving hematopoietic stem-cell maintenance via AKT pathway modulation, and serving as an essential, druggable node whose loss-of-function triggers ribosomal stress and selective apoptosis in acute myeloid leukemia cells.
免疫印迹
WB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDaWB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDaWB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDaWB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CK1α in tumor tissue.
Expression of CK1α in human tissue.
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