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产品中心 抗体 病理/IHC抗体

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ER alpha Recombinant Rabbit mAb, PBS Only (SDT-2950-36)

Estrogen receptor,ER,ER-alpha,Estradiol receptor,Nuclear receptor subfamily 3 group A member 1,ESR,NR3A1,ESR1

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货号 S0B2444P
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产品介绍

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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ER alpha

  • 分子别名

    Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR; NR3A1; ESR1

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm, Nucleus, Cell membrane

  • Accession

    P03372

  • 克隆号

    SDT-2950-36

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 阳性样本

    MCF7, T-47D

  • 纯化方式

    Protein A

  • 浓度

    1 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:500 Hu
    IHC-P 1:250 Hu
    ICC 1:500 Hu
背景介绍

  • ER alpha (estrogen receptor alpha) is a 66-kDa ligand-activated transcription factor belonging to the nuclear receptor superfamily that, upon binding 17β-estradiol, translocates to the nucleus, homodimerizes, and recruits co-regulators to estrogen response elements (EREs) in target genes, thereby governing proliferation, differentiation, and survival in reproductive tissues, bone, cardiovascular system, and CNS, while its dysregulation drives breast and endometrial cancers, making it the principal therapeutic target of selective modulators (SERMs) and degraders (SERDs).

  • 免疫印迹

    • WB result of ER alpha Recombinant Rabbit mAb
      Primary antibody: ER alpha Recombinant Rabbit mAb at 1/500 dilution
      Lane 1: SK-BR-3 whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: T-47D whole cell lysate 20 µg
      Negative control: SK-BR-3 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 66 kDa
      Observed MW: 66 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human breast. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer (case 1). Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer (case 2). Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in T-47D cells (top panel) and negative staining in SK-BR-3 cells (below panel). Anti-ER alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of ER alpha in tumor tissue.

    • Expression of ER alpha in human tissue.

    • Expression of ER alpha in breast cancer TMA.

    • Expression of ER alpha in ovarian cancer TMA.

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