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Rabbit抗原名称
ER alpha分子别名
Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR; NR3A1; ESR1免疫原
Recombinant Protein细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
P03372克隆号
SDT-2950-36抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, T-47D纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500 Hu IHC-P 1:250 Hu ICC 1:500 Hu
ER alpha (estrogen receptor alpha) is a 66-kDa ligand-activated transcription factor belonging to the nuclear receptor superfamily that, upon binding 17β-estradiol, translocates to the nucleus, homodimerizes, and recruits co-regulators to estrogen response elements (EREs) in target genes, thereby governing proliferation, differentiation, and survival in reproductive tissues, bone, cardiovascular system, and CNS, while its dysregulation drives breast and endometrial cancers, making it the principal therapeutic target of selective modulators (SERMs) and degraders (SERDs).
免疫印迹
WB result of ER alpha Recombinant Rabbit mAb
Primary antibody: ER alpha Recombinant Rabbit mAb at 1/500 dilution
Lane 1: SK-BR-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Negative control: SK-BR-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 66 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer (case 1). Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer (case 2). Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in T-47D cells (top panel) and negative staining in SK-BR-3 cells (below panel). Anti-ER alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ER alpha in tumor tissue.
Expression of ER alpha in human tissue.
Expression of ER alpha in breast cancer TMA.
Expression of ER alpha in ovarian cancer TMA.
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