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Rabbit抗原名称
Napsin A分子别名
Napsin-A; Aspartyl protease 4 (ASP4; Asp 4); Napsin-1; TA01/TA02; NAPSA; NAP1; NAPA免疫原
Recombinant Protein细胞定位
SecretedAccession
O96009克隆号
SDT-2777-115抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HCC78, HCC827纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:250 Hu IHC-P 1:5000 Hu
Napsin A is a pepsin-like aspartic proteinase predominantly expressed in type II pneumocytes and renal tubular cells; it is widely used as an immunohistochemical marker because its strong cytoplasmic staining is present in ~85 % of lung adenocarcinomas while being virtually absent in squamous cell carcinomas, making it valuable for distinguishing primary pulmonary adenocarcinoma from other tumor types.
免疫印迹
WB result of Napsin A Recombinant Rabbit mAb
Primary antibody: Napsin A Recombinant Rabbit mAb at 1/250 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HCC78 whole cell lysate 20 µg
Lane 3: HCC827 whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 30-40 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human lung. Anti-Napsin A antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Napsin A antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Napsin A antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Napsin A antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Napsin A antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Napsin A in tumor tissue.
Expression of Napsin A in human tissue.
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