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CD5 Recombinant Rabbit mAb (SDT-2857-16)

T-cell surface glycoprotein CD5,Lymphocyte antigen T1/Leu-1,LEU1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2441
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    CD5

  • 分子别名

    T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; LEU1

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cell membrane

  • Accession

    P06127

  • 克隆号

    SDT-2857-16

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:500 Hu, Ms
背景介绍

  • CD5 is a 67 kDa type I cell surface glycoprotein that belongs to the scavenger receptor cysteine-rich (SRCR) family. It is expressed on all T cells and a subset of B cells known as B-1a cells. CD5 plays a crucial role in regulating T cell receptor (TCR) signaling and acts as a negative regulator of T cell activation. It helps fine-tune the threshold for T cell activation during thymocyte development and suppresses peripheral T cell activation by inhibiting TCR-proximal signaling. In B-1a cells, CD5 helps prevent self-reactivity by dampening B cell receptor (BCR) signals. The expression of CD5 is also associated with cell survival, apoptosis, and the development of regulatory T cells. Additionally, CD5 may be involved in the regulation of dendritic cell function and cytokine production.

  • 免疫印迹

    • WB result of CD5 Recombinant Rabbit mAb
      Primary antibody: CD5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Ramos whole cell lysate 20 µg
      Lane 2: Raji whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: Jurkat whole cell lysate 20 µg
      Lane 5: Molt-4 whole cell lysate 20 µg
      Negative control: Ramos whole cell lysate; Raji whole cell lysate; HeLa whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 55 kDa

    • WB result of CD5 Recombinant Rabbit mAb
      Primary antibody: CD5 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 65 kDa

  • 免疫沉淀

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-CD5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CD5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of CD5 in tumor tissue.

    • Expression of CD5 in human tissue.

    • Expression of CD5 in mouse & rat tissue.

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