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产品中心 抗体 病理/IHC抗体

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Cardiac troponin T (TNNT2) Recombinant Rabbit mAb (SDT-2894-34)

Troponin T,cardiac muscle,TnTc,Cardiac muscle troponin T (cTnT)

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2440
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Cardiac troponin T (TNNT2)

  • 分子别名

    Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T (cTnT)

  • 免疫原

    Recombinant Protein

  • Accession

    P45379

  • 克隆号

    SDT-2894-34

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    IF 1:250 Hu
背景介绍

  • Cardiac troponin T (TNNT2) is a 35.9 kDa, 295-amino-acid striated-muscle-specific protein that forms the troponin T component of the troponin complex on the thin filament, where it tethers the troponin C–I sub-complex to tropomyosin; by transducing the Ca²⁺-induced conformational change of troponin C into tropomyosin movement, TNNT2 orchestrates the steric switch that exposes myosin-binding sites on actin and thus initiates systolic contraction, while its unique N-terminal peptide sequence allows ultra-sensitive immunoassay detection—making any elevation in blood TNNT2 the gold-standard biomarker for myocardial injury—yet mutations that enhance or suppress myofilament Ca²⁺ sensitivity, destabilize the N-tail, or promote aberrant splice variants are linked to hypertrophic, dilated, or restrictive cardiomyopathies, arrhythmogenic right ventricular cardiomyopathy, and sudden cardiac death, underscoring its dual role as both a molecular motor controller and a sentinel of cardiac integrity.

  • 免疫印迹

    • WB result of Cardiac troponin T (TNNT2) Recombinant Rabbit mAb
      Primary antibody: Cardiac troponin T (TNNT2) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse skeletal muscle lysate 20 µg
      Lane 2: mouse heart lysate 20 µg
      Negative control: mouse skeletal muscle lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 39 kDa

    • WB result of Cardiac troponin T (TNNT2) Recombinant Rabbit mAb
      Primary antibody: Cardiac troponin T (TNNT2) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat skeletal muscle lysate 20 µg
      Lane 2: rat heart lysate 20 µg
      Negative control: rat skeletal muscle lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 39 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-Cardiac troponin T (TNNT2) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human cardiac muscle. Anti- Cardiac troponin T (TNNT2) antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded human skeletal muscle. Anti- Cardiac troponin T (TNNT2) antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of Cardiac troponin T (TNNT2) in human tissue.

    • Expression of Cardiac troponin T (TNNT2) in mouse & rat tissue.

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