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抗原名称
Angiotensin Converting Enzyme 1分子别名
Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD143; DCP; DCP1免疫原
Recombinant Protein细胞定位
Cytoplasm, Secreted, Cell membraneAccession
P12821克隆号
SDT-2662-122抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:2000 Hu, Ms, Rt IF 1:500 Hu, Ms, Rt
Angiotensin-Converting Enzyme 1 (ACE1) is a zinc-dependent dipeptidyl carboxypeptidase expressed as a 1,306-amino-acid type-I transmembrane glycoprotein, with the bulk of its structure (residues 1–1230) projecting into the extracellular space and containing two homologous catalytic N- and C-terminal domains, each bearing the characteristic HEXXH zinc-binding motif; by cleaving the decapeptide angiotensin I into the potent vasoconstrictor octapeptide angiotensin II, degrading the vasodilator bradykinin, and modulating additional vasoactive peptides, ACE1 sits at the apex of the renin–angiotensin–aldosterone system, thereby governing systemic blood pressure, extracellular fluid volume, and cardiovascular remodeling, while its genetic polymorphisms—most notably the Alu insertion/deletion (I/D) variant in intron 16—have been linked to inter-individual differences in circulating enzyme levels and risk for hypertension, myocardial infarction, diabetic nephropathy, and COVID-19 severity, making ACE1 a cornerstone target for therapeutic inhibitors such as captopril, enalapril, and lisinopril that are now mainstays in treating hypertension and heart failure.
免疫印迹
WB result of Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb
Primary antibody: Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDaWB result of Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb
Primary antibody: Angiotensin Converting Enzyme 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Angiotensin Converting Enzyme 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human kidney. Anti- Angiotensin Converting Enzyme 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse kidney. Anti- Angiotensin Converting Enzyme 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat lung. Anti- Angiotensin Converting Enzyme 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Angiotensin Converting Enzyme 1 in tumor tissue.
Expression of Angiotensin Converting Enzyme 1 in human tissue.
Expression of Angiotensin Converting Enzyme 1 in mouse & rat tissue.
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