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Rabbit抗原名称
ERCC1分子别名
DNA excision repair protein ERCC-1免疫原
Recombinant Protein细胞定位
Cytoplasm, NucleusAccession
P07992克隆号
SDT-2458-29抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HepG2, SK-BR-3, MCF7, T-47D纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:2000 Hu ICFCM 1:200 Hu
ERCC1 (Excision Repair Cross-Complementation Group 1) is a 297-amino-acid protein that, together with its obligate partner XPF (ERCC4), forms a structure-specific heterodimeric endonuclease essential for nucleotide excision repair (NER) of helix-distorting DNA lesions caused by UV light or bulky adducts and for interstrand crosslink repair; the ERCC1–XPF complex incises the damaged DNA strand on the 5′ side of the lesion, enabling excision of a ~24–32-nt oligonucleotide, and its expression level or polymorphisms (e.g., C118T, C8092A) are extensively studied as predictive biomarkers for resistance to platinum-based chemotherapy in lung, ovarian, and other solid tumors because reduced ERCC1-mediated DNA repair enhances platinum-induced cytotoxicity.
免疫印迹
WB result of ERCC1 Recombinant Rabbit mAb
Primary antibody: ERCC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SK-BR-3 (Human breast adenocarcinoma epithelial cell) labelling ERCC1 antibody at 1/200 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SK-BR-3 cells. Anti-ERCC1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ERCC1 in tumor tissue.
Expression of ERCC1 in human tissue.
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