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宿主来源
Rabbit抗原名称
Fumarate hydratase分子别名
Fumarate hydratase, mitochondrial; Fumarase; HsFH; FH免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, MitochondrionAccession
P07954克隆号
SDT-2434-37抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HEK-293, HepG2, MCF7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
Fumarate hydratase (FH) is a mitochondrial and cytoplasmic metabolic enzyme encoded by the FH gene that catalyzes the reversible stereospecific hydration of fumarate to L-malate within the tricarboxylic acid cycle, a reaction essential for NADH production and cellular energy homeostasis; beyond this canonical metabolic role, FH acts as a tumor suppressor because loss-of-function mutations lead to intracellular accumulation of the oncometabolite fumarate, which inhibits α-ketoglutarate-dependent dioxygenases (including HIF prolyl-hydroxylases and histone demethylases), thereby stabilizing HIF-1α, driving pseudohypoxic gene expression, succinating KEAP1 to activate Nrf2 antioxidant signaling, and ultimately promoting hereditary leiomyomatosis and renal cell carcinoma, while complete FH deficiency causes the severe neurometabolic disorder fumaric aciduria characterized by encephalopathy, developmental delay, and elevated fumarate levels, and the enzyme also moonlights in DNA repair by translocating to double-strand breaks where locally generated fumarate enhances non-homologous end-joining through inhibition of KDM2B and increased H3K36 dimethylation.
免疫印迹
WB result of Fumarate hydratase Recombinant Rabbit mAb
Primary antibody: Fumarate hydratase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 46 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Fumarate hydratase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Fumarate hydratase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Fumarate hydratase in tumor tissue.
Expression of Fumarate hydratase in human tissue.
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