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宿主来源
Rabbit抗原名称
MUC5AC分子别名
Mucin-5AC; MUC-5AC Curated; Gastric mucin; Major airway glycoprotein; Mucin-5 subtype AC; tracheobronchial; Tracheobronchial mucin (TBM); MUC5免疫原
Recombinant Protein细胞定位
SecretedAccession
P98088克隆号
SDT-2630-23抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A549纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000-1:2000 Hu ICC 1:500 Hu IF 1:500 Hu
MUC5AC is a gel-forming mucin glycoprotein secreted by goblet cells of the respiratory and gastrointestinal tracts, forming a viscous layer that protects epithelial surfaces by trapping inhaled pathogens and particulates, and its overexpression is a hallmark of mucus hypersecretion diseases such as asthma, COPD and cystic fibrosis.
免疫印迹
WB result of MUC5AC Recombinant Rabbit mAb
Primary antibody: MUC5AC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 585 kDa
Observed MW: 130-600 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MUC5AC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A549 cells (top panel) and negative staining in HeLa cells (below panel). Anti-MUC5AC antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded human stomach. Anti-MUC5AC antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of MUC5AC in tumor tissue.
Expression of MUC5AC in human tissue.
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