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宿主来源
Rabbit抗原名称
LY9/CD229分子别名
T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3 (SLAMF3); Signaling lymphocytic activation molecule 3免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q9HBG7克隆号
SDT-2393-47抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Daudi, Jurkat纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu
CD229, also known as LY9 or SLAMF3, is a 120 kDa type I transmembrane glycoprotein of the SLAM subgroup of the CD2 family, expressed on T, B, NK, dendritic and stem cells, that functions as a self-ligand through homophilic binding of its N-terminal IgV-like domain to regulate immune responses. Upon engagement, phosphorylation of two ITSM motifs in its long cytoplasmic tail recruits the adaptor proteins SAP or EAT-2 (or the phosphatases SHP-2/SHIP1), leading to signals that can either enhance or inhibit leukocyte activation, differentiation, cytokine production and cytotoxicity, depending on cellular context, and thereby controls processes such as T-cell adhesion and immunological synapse formation, Th17 polarization, maintenance of peripheral tolerance and prevention of autoimmunity, while its overexpression on malignant plasma and memory B cells has made CD229 a prognostic biomarker and CAR-T target in multiple myeloma and other B-cell malignancies.
免疫印迹
WB result of LY9/CD229 Recombinant Rabbit mAb
Primary antibody: LY9/CD229 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: U937 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 60-130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human myeloma. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of LY9/CD229 in tumor tissue.
Expression of LY9/CD229 in human tissue.
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