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Rabbit抗原名称
LICAM分子别名
Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; CAML1; MIC5; L1CAM免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P32004克隆号
SDT-2594-39抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu IHC-P 1:250 Hu
LICAM (L1 cell adhesion molecule) is a 200–220 kDa transmembrane glycoprotein of the immunoglobulin superfamily that functions as a pivotal regulator of neural development—guiding neuronal migration, axon outgrowth and fasciculation via homophilic and heterophilic interactions with integrins and other adhesion receptors—and, when re-expressed in cancers such as glioblastoma, lung, endometrial and renal carcinomas, is proteolytically cleaved into soluble ectodomain and cytoplasmic fragments that activate integrin–FAK–GRB2–SOS–RAS signaling, thereby enhancing tumor-cell motility, invasion, trans-endothelial migration and metastasis and serving as an independent marker of poor prognosis and a potential therapeutic target.
免疫印迹
WB result of LICAM Recombinant Rabbit mAb
Primary antibody: LICAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Negative control: U-87 MG whole cell lysate; LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 140 kDa
Observed MW: 280 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-LICAM antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of LICAM in tumor tissue.
Expression of LICAM in human tissue.
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