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产品中心 抗体 病理/IHC抗体

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TCF-4 Recombinant Rabbit mAb (SDT-2549-4)

Transcription factor 4,Class B basic helix-loop-helix protein 19 (bHLHb19),Immunoglobulin transcription factor 2 (ITF-2),SL3-3 enhancer factor 2 (SEF-2),BHLHB19,ITF2,SEF2,TCF4

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2428
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    TCF-4

  • 分子别名

    Transcription factor 4; Class B basic helix-loop-helix protein 19 (bHLHb19); Immunoglobulin transcription factor 2 (ITF-2); SL3-3 enhancer factor 2 (SEF-2); BHLHB19; ITF2; SEF2; TCF4

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P15884

  • 克隆号

    SDT-2549-4

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Daudi, SH-SY5Y, Neruo-21, A20

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:250 Hu, Ms, Rt
    ICC 1:50 Hu, Ms
背景介绍

  • TCF-4 (Transcription Factor 4), a 667-amino-acid basic helix–loop–helix protein encoded by the TCF4 gene on chromosome 18q21.2, is a master regulator of neurodevelopment, immune tolerance and epithelial homeostasis that binds the canonical E-box motif 5′-CACCTG-3′ to activate or repress transcription in partnership with β-catenin in the Wnt pathway, interacts with HDACs and CBP/p300 to modulate chromatin state, and whose dosage-sensitive expression is critically linked to Pitt–Hopkins syndrome and schizophrenia while its somatic amplifications and activating mutations drive colorectal, pancreatic and breast tumorigenesis by enforcing a stem-like transcriptional program.

  • 免疫印迹

    • WB result of TCF-4 Recombinant Rabbit mAb
      Primary antibody: TCF-4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Daudi whole cell lysate 20 µg
      Lane 2: SH-SY5Y whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 71 kDa
      Observed MW: 55-65 kDa
      This blot was developed with high sensitivity substrate

    • WB result of TCF-4 Recombinant Rabbit mAb
      Primary antibody: TCF-4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: A20 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 71 kDa
      Observed MW: 55-80 kDa
      This blot was developed with high sensitivity substrate

  • 免疫沉淀

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in SH-SY5Y cells. Anti- TCF-4 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in Neuro-2a cells. Anti- TCF-4 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of TCF-4 in tumor tissue.

    • Expression of TCF-4 in human tissue.

    • Expression of TCF-4 in mouse & rat tissue.

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