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宿主来源
Mouse抗原名称
FOXP3分子别名
Forkhead box protein P3; Scurfin; IPEX细胞定位
Cytoplasm, NucleusAccession
Q9BZS1克隆号
SDT-3494抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms, Rt阳性样本
mouse heart纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IHC-P 1:250-1:1000 Hu, Ms, Rt
FOXP3 protein is a crucial transcription factor that plays a central role in the development and function of regulatory T cells (Tregs), which are essential for maintaining immune tolerance and preventing autoimmune diseases. It is predominantly expressed in Tregs and helps suppress the activity of other immune cells, thereby preventing excessive immune responses. Mutations or dysfunction in the FOXP3 gene can lead to severe autoimmune disorders such as IPEX syndrome, highlighting its importance in immune regulation. Additionally, FOXP3 has been implicated in various other biological processes, including cancer immunology, where its expression in tumor-infiltrating Tregs can influence the tumor microenvironment and affect the efficacy of immunotherapies.
免疫印迹
WB result of FOXP3 Recombinant Mouse mAb
Primary antibody: FOXP3 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXP3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-FOXP3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-FOXP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-FOXP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FOXP3 in tumor tissue.
Expression of FOXP3 in human tissue.
Expression of FOXP3 in mouse & rat tissue.
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