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Rabbit抗原名称
VGLUT3分子别名
Vesicular glutamate transporter 3; VGluT3; Solute carrier family 17 member 8; Slc17a8免疫原
Recombinant Protein细胞定位
Cell membrane, SynapseAccession
Q8BFU8克隆号
S-2430-26抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
Mouse hippocampus, mouse brain, rat hippocampus, rat brain纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:250 Ms IHC-P 1:500 Ms, Rt IF 1:500 Ms
VGLUT3 (Vesicular Glutamate Transporter 3) is a 60-kDa multi-pass transmembrane protein encoded by the SLC17A8 gene that packages glutamate into synaptic vesicles for activity-dependent release, but unlike VGLUT1 and VGLUT2 it is expressed primarily in neurons that were traditionally considered non-glutamatergic—such as cholinergic striatal interneurons, serotonergic raphe neurons, and GABAergic interneurons—thereby enabling co-transmission of glutamate with acetylcholine, serotonin, or GABA; VGLUT3 also localizes to the somatodendritic compartment where it can modulate retrograde signaling, and genetic deletion in mice produces auditory neuropathy, social deficits, and altered pain perception, while human SLC17A8 mutations cause progressive sensorineural deafness (DFNA25), collectively illustrating its critical role in diverse circuits ranging from cochlear ribbon synapses to cortico-striatal networks.
免疫印迹
WB result of VGLUT3 Recombinant Rabbit mAb
Primary antibody: VGLUT3 Recombinant Rabbit mAb at 1/250 dilution
Lane 1: unboiled mouse hippocampus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded mouse hippocampus. Anti-VGLUT3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VGLUT3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat hippocampus. Anti-VGLUT3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VGLUT3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-VGLUT3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse hippocampus. Anti-VGLUT3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse brain. Anti-VGLUT3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded mouse hippocampus. Anti-VGLUT3 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of VGLUT3 in mouse & rat tissue.
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