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宿主来源
Mouse抗原名称
Cytokeratin 6A (KRT6A)分子别名
Keratin, type II cytoskeletal 6A; Cytokeratin-6A (CK-6A); Cytokeratin-6D (CK-6D); Keratin-6A (K6A); Type-II keratin Kb6; Allergen name; Hom s 5; K6A; KRT6D细胞定位
Nucleus, MembraneAccession
P02538克隆号
SDT-3380抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu, Ms阳性样本
A431, HaCaT纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu IHC-P 1:1000 Hu, Ms
Cytokeratin 6A (KRT6A) is a type II intermediate filament keratin that assembles with its obligatory partner keratin 16 to form robust cytoskeletal networks in stratified epithelia, especially in palmoplantar epidermis and oral mucosa, where it confers mechanical resilience under shear stress; its expression is rapidly up-regulated following injury, inflammation, or hyperproliferation via STAT3, NF-κB and MAPK signaling, and gain-of-function mutations (e.g., p.N171K) cause pachyonychia congenita with thickened nails and painful palmoplantar keratoderma, while loss-of-function variants delay wound closure and attenuate keratinocyte migration, highlighting KRT6A’s dual role in epithelial protection and pathological remodeling.
免疫印迹
WB result of Cytokeratin 6A (KRT6A) Recombinant Mouse mAb
Primary antibody: Cytokeratin 6A (KRT6A) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HaCaT whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Cytokeratin 6A (KRT6A) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skin. Anti-Cytokeratin 6A (KRT6A) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Cytokeratin 6A (KRT6A) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Cytokeratin 6A (KRT6A) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human prostatic cancer. Anti-Cytokeratin 6A (KRT6A) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse esophagus. Anti-Cytokeratin 6A (KRT6A) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Cytokeratin 6A (KRT6A) in tumor tissue.
Expression of Cytokeratin 6A (KRT6A) in human tissue.
Expression of Cytokeratin 6A (KRT6A) in mouse & rat tissue.
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