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宿主来源
Rabbit抗原名称
UCP1分子别名
Mitochondrial brown fat uncoupling protein 1; UCP 1 Curated; Solute carrier family 25 member 7; Thermogenin; SLC25A7; UCP免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P25874克隆号
SDT-2275-37抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
human brown adipose, mouse brown adipose预测反应种属
(反应种属缩写表)Sq, Hm纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Ms IHC-P 1:250 Hu, Ms IF 1:500 Ms
UCP1 (uncoupling protein 1), a 33 kDa integral membrane protein located in the inner mitochondrial membrane of brown adipose tissue, operates as a protonophore that dissipates the electrochemical gradient generated by the electron transport chain, thereby uncoupling oxidative phosphorylation from ATP synthesis and converting the released chemical energy into heat (non-shivering thermogenesis); it is activated by cold-induced sympathetic release of norepinephrine via β3-adrenergic receptors, which elevates cAMP and triggers lipolysis, liberating free fatty acids that both allosterically activate UCP1 and serve as respiratory substrates, while purine nucleotides (ATP/ADP) inhibit its activity competitively, and its transcription is synergistically regulated by PGC-1α, PPARγ, and the thyroid hormone T3, making UCP1 a critical determinant of systemic energy expenditure and a promising target for anti-obesity therapeutics.
免疫印迹
WB result of UCP1 Recombinant Rabbit mAb
Primary antibody: UCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse adipose lysate 20 µg
Lane 2: mouse brown adipose lysate 20 µg
Negative control: mouse adipose lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 32 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse brown adipose. Anti-UCP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse white adipose. Anti-UCP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse brown adipose. Anti-UCP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded mouse white adipose. Anti-UCP1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of UCP1 in tumor tissue.
Expression of UCP1 in human tissue.
Expression of UCP1 in mouse & rat tissue.
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