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宿主来源
Rabbit抗原名称
Wnt3a分子别名
Protein Wnt-3a; WNT3A细胞定位
SecretedAccession
P56704克隆号
S-3300抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:20000 Ms IHC-P 1:200 Ms ICC 1:200 Ms
Wnt3a is a secreted, palmitoleated glycoprotein of ~39 kDa that acts as a principal ligand for canonical Wnt signaling; upon binding to Frizzled and LRP5/6 co-receptors it triggers a β-catenin–dependent cascade leading to TCF/LEF-mediated transcription of genes that govern embryonic axis formation, neural crest specification, self-renewal of hematopoietic and intestinal stem cells, and osteoblast differentiation, while its dysregulation drives colorectal, breast and hematologic malignancies, and recombinant Wnt3a is widely used in vitro to expand organoids and maintain pluripotency.
免疫印迹
WB result of Wnt3a Recombinant Rabbit mAb
Primary antibody: Wnt3a Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: L Wnt-3A whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded E14.5 mouse embryo. Anti-Wnt3a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in L Wnt-3A cells. Anti-Wnt3a antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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