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宿主来源
Mouse抗原名称
TLR4分子别名
Toll-like receptor 4; CD284; Lps; Tlr4细胞定位
Endosome, Cell membraneAccession
Q9QUK6克隆号
SDT-3305抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Pig纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:250 Hu
Toll-like receptor 4 (TLR4) is a type I transmembrane pattern-recognition receptor expressed predominantly on macrophages, dendritic cells, and epithelial barriers; upon recognition of lipopolysaccharide from Gram-negative bacteria in complex with MD-2 and CD14, it homodimerizes and initiates a MyD88- and TRIF-dependent signaling cascade that activates NF-κB, MAPKs, and IRF3, driving the transcription of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), type I interferons, and co-stimulatory molecules essential for innate immunity and the subsequent shaping of adaptive responses, while excessive or dysregulated TLR4 activation contributes to septic shock, chronic inflammatory disorders such as atherosclerosis and metabolic syndrome, and ischemia–reperfusion injury, making it a therapeutic target for antagonists like lipid A analogs and monoclonal antibodies.
免疫组化
IHC shows positive staining in paraffin-embedded human skin. Anti-TLR4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human salivary gland. Anti-TLR4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-TLR4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TLR4 in tumor tissue.
Expression of TLR4 in human tissue.
Expression of TLR4 in mouse & rat tissue.
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