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Rabbit抗原名称
MelanA分子别名
Melanoma antigen recognized by T-cells 1; MART-1; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A; MART1; MLANA细胞定位
Endoplasmic reticulum, CytoplasmAccession
Q16655克隆号
SDT-3352抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
SK-MEL-28, B16F0纯化方式
Protein A浓度
1mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms IHC-P 1:1000 Hu, Ms ICC 1:500 Hu, Ms
MelanA (also called MART-1) is a 13 kDa single-pass transmembrane protein encoded by the MLANA gene, expressed almost exclusively in melanocytes of the skin and retina, where it stabilizes and traffics the melanosomal glycoprotein gp100 to facilitate stage II melanosome biogenesis and melanin synthesis; its immunogenic 26-35 amino-acid fragment is presented on HLA class I molecules and constitutes one of the most frequent targets of circulating naïve CD8⁺ T cells, making the protein both a key histopathologic marker (detected by antibodies A103 or M2-7C10) for melanocytic tumors and a central component in experimental melanoma vaccines .
免疫印迹
WB result of MelanA Recombinant Rabbit mAb
Primary antibody: MelanA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: OVCAR-3 whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Negative control: OVCAR-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 20 kDaWB result of MelanA Recombinant Rabbit mAb
Primary antibody: MelanA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: B16F0 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 20 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human melanoma (case 1). Anti-MelanA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human melanoma (case 2). Anti-MelanA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-MelanA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-MelanA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-MelanA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in B16F0 cells. Anti-MelanA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in SK-MEL-28 cells (top panel) and negative staining in OVCAR3 cells (below panel). Anti-MelanA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of MelanA in tumor tissue.
Expression of MelanA in human tissue.
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