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宿主来源
Rabbit抗原名称
TOMM20分子别名
Mitochondrial import receptor subunit TOM20 homolog; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20; KIAA0016免疫原
Recombinant Protein细胞定位
MitochondrionAccession
Q15388克隆号
SDT-2553-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, HeLa, SH-SY5Y, NIH/3T3, PC-12, mouse kidney, mouse heart, mouse skeletal muscle, PC-12, rat kidney, rat heart, rat skeletal muscle预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
1mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms, Rt
TOM20 (Translocase of Outer Mitochondrial Membrane 20) is a ~20 kDa receptor subunit of the TOM complex embedded in the outer mitochondrial membrane; together with TOM20 it forms the initial binding site that recognizes N-terminal matrix-targeting sequences on cytosol-synthesized pre-proteins and threads them through the TOMM40 channel into mitochondria, thereby governing mitochondrial biogenesis and bioenergetics, while also moonlighting as a mitophagy adaptor via PINK1, a regulator of apoptosis through BCL2/BAX, and a chaperone-like stabilizer of the androgen receptor in prostate cancer, making it a widely used marker for mitochondrial mass and a potential biomarker for diseases such as Parkinson’s and cancer .
免疫印迹
WB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDaWB result of T TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Lane 4: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDaWB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat heart lysate 20 µg
Lane 4: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-TOM20 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-TOM20 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TOM20 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-TOM20 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-TOM20 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-TOM20 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in RAW264.7 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in PC-12 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TOM20 in tumor tissue.
Expression of TOM20 in human tissue.
Expression of TOM20 in mouse & rat tissue.
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