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Rabbit抗原名称
Somatostatin Receptor 2分子别名
Somatostatin receptor type 2; SS-2-R; SS2-R; SS2R; SST2; SRIF-1; SSTR2免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P30874克隆号
SDT-2507-1抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Pg,Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:250-1:1000 Hu, Ms, Rt IF 1:100-1:500 Hu, Ms
Somatostatin receptor 2 (SSTR2), encoded by the SSTR2 gene on chromosome 17q25.1, is a 369-amino-acid Gi-coupled class A G-protein-coupled receptor (GPCR) with seven transmembrane helices, extracellular N-glycosylation sites and intracellular phosphorylation motifs, existing as two splice variants (SST2A/B) with SST2A predominating in human tissues; it is highly expressed in brain and endocrine organs (pancreatic α/β-cells, pituitary somatotrophs) where it binds somatostatin-14/28 to inhibit adenylyl cyclase, voltage-gated Ca²⁺ channels and hormone secretion (e.g., glucagon, insulin, GH, TSH), and is widely over-expressed in neuroendocrine tumors, making it both a prognostic marker and a therapeutic target for radiolabeled peptides or selective agonists such as octreotide to suppress tumor growth and induce apoptosis .
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas. Anti-Somatostatin Receptor 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Somatostatin Receptor 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Somatostatin Receptor 2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-Somatostatin Receptor 2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-Somatostatin Receptor 2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human pancreas. Anti- Somatostatin Receptor 2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse pancreas. Anti- Somatostatin Receptor 2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Somatostatin Receptor 2 in tumor tissue.
Expression of Somatostatin Receptor 2 in human tissue.
Expression of Somatostatin Receptor 2 in mouse & rat tissue.
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