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Rabbit抗原名称
Myelin protein zero分子别名
Myelin protein P0; Myelin peripheral protein (MPP); MPZ免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P25189克隆号
SDT-1225-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
Human sciatic nerve, mouse sciatic nerve预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IHC-P 1:1000 Hu, Ms, Rt IF 1:500 Hu
Myelin protein zero (P0, MPZ) is a 28–30 kDa type I transmembrane glycoprotein that constitutes 50–70 % of total protein in peripheral nervous system (PNS) compact myelin, where it is expressed continuously by myelinating Schwann cells until mature myelin is formed; its extracellular immunoglobulin-like domain mediates homophilic adhesion between apposing membrane pairs to generate the characteristic intraperiod line, while its short cytoplasmic tail cooperates with myelin basic protein to zipper the cytoplasmic faces, enabling the tight membrane stacking that gives PNS myelin its insulating properties, and mutations in the MPZ gene underlie a spectrum of incurable demyelinating peripheral neuropathies such as Charcot–Marie–Tooth disease by disrupting these adhesive and structural functions.
免疫印迹
WB result of Myelin protein zero Recombinant Rabbit mAb
Primary antibody: Myelin protein zero Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse sciatic nerve lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human sciatic nerve of foot. Anti-Myelin protein zero antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Myelin protein zero antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-Myelin protein zero antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Myelin protein zero antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Myelin protein zero antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human sciatic nerve of foot. Anti-Myelin protein zero antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Myelin protein zero in tumor tissue.
Expression of Myelin protein zero in human tissue.
Expression of Myelin protein zero in mouse & rat tissue.
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