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宿主来源
Rabbit抗原名称
FOXL2分子别名
Forkhead box protein L2免疫原
Synthetic Peptide细胞定位
NucleusAccession
P58012克隆号
SDT-557-361抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
K562, A549, mouse ovary纯化方式
Protein A浓度
1mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4°C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu IF 1:500 Ms
FOXL2 is a winged-helix/forkhead transcription factor encoded on chromosome 3q23 that is indispensable for female sex determination, ovarian somatic-cell differentiation, granulosa-cell function, and eyelid morphogenesis; mutations or deletions in FOXL2 cause blepharophimosis–ptosis–epicanthus inversus syndrome and are pathognomonically associated with adult granulosa-cell tumours, while somatic missense mutations (most prominently C134W) constitutively activate the protein, driving oncogenesis through altered transcriptional programs that maintain granulosa-cell lineage identity and suppress apoptosis, thereby highlighting its dual role as a gatekeeper of ovarian development and a lineage-specific tumour suppressor/oncogene.
免疫印迹
WB result of FOXL2 Recombinant Rabbit mAb
Primary antibody: FOXL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrateWB result of FOXL2 Recombinant Rabbit mAb
Primary antibody: FOXL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse ovary lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human ovary. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian granulosa cell tumor. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse ovary. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat ovary. Anti-FOXL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A549 cells (top panel) and negative staining in MCF7 cells (below panel). Anti-FOXL2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse ovary. Anti-FOXL2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of FOXL2 in tumor tissue.
Expression of FOXL2 in human tissue.
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