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产品中心 抗体 病理/IHC抗体

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ATP4B Recombinant Rabbit mAb,PBS Only (SDT-2445-64)

Potassium-transporting ATPase subunit beta,Gastric H(+)/K(+) ATPase subunit beta,Proton pump beta chain

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货号 S0B2404P
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ATP4B

  • 分子别名

    Potassium-transporting ATPase subunit beta; Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    P51164

  • 克隆号

    SDT-2445-64

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    1 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied

  • 应用

    IHC-P ?

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    IHC-P 1:1000-1:2000 Hu
    IF 1:250-1:500 Hu
背景介绍

  • ATP4B (ATPase H+/K+ transporting subunit beta) is a 291-amino-acid, heavily glycosylated transmembrane protein that forms the regulatory β-subunit of the heterodimeric gastric H+/K+-ATPase proton pump, which exchanges cytoplasmic H+ for extracellular K+ across the apical membrane of parietal cells to drive gastric acid secretion; it stabilizes and targets the catalytic α-subunit to the membrane, acts as a conformational ratchet that locks the pump in its outward-facing E2 state, and contributes to epithelial cell polarity and adhesion through an immunoglobulin-like C-terminal ectodomain .

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-ATP4B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human gastric cancer. Anti-ATP4B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human stomach. Anti-ATP4B antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of ATP4B in tumor tissue.

    • Expression of ATP4B in human tissue.

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