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产品中心 抗体 病理/IHC抗体

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CRX Recombinant Rabbit mAb, PBS Only (SDT-2194-105)

Cone-rod homeobox protein,CORD2

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货号 S0B2403P
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CRX

  • 分子别名

    Cone-rod homeobox protein; CORD2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    O43186

  • 克隆号

    SDT-2194-105

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Bv, Dg, Gor, Cz, GP

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS

  • 储存条件

    12 months from date of receipt / reconstitution,4 °C as supplied

  • 应用

    IHC-P ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Ms
    IHC-P 1:1000 Hu, Ms, Rt
    IF 1:100 Ms
背景介绍

  • CRX (Cone-Rod Homeobox) is a 299-amino-acid photoreceptor-specific transcription factor of the OTX-like homeodomain family, encoded by the CRX gene on 19q13.32, that binds to and trans-activates promoters and enhancers of rod and cone photoreceptor genes—including rhodopsin, OPN1SW, OPN1MW, PDE6 subunits and arrestin—via the PCE (photoreceptor conserved element) motif, thereby orchestrating the development, differentiation and daily maintenance of outer-segment structure and phototransduction; autosomal-dominant mutations—missense, truncating, or C-terminal frameshift—cause a spectrum of retinal dystrophies such as cone-rod dystrophy-2, Leber congenital amaurosis-7 and retinitis pigmentosa, while CRX haploinsufficiency or dominant-negative interference disrupts transcriptional networks synergistic with OTX2 and NRL, leading to progressive photoreceptor degeneration and visual impairment.

  • 免疫印迹

    • WB result of CRX Recombinant Rabbit mAb
      Primary antibody: CRX Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse kidney lysate 20 µg
      Lane 2: mouse eye lysate 20 µg
      Negative control: mouse kidney lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 32 kDa
      Observed MW: 37 kDa
      This blot was developed with high sensitivity substrate

  • 免疫沉淀

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human retina. Anti-CRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse retina. Anti-CRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-CRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat retina. Anti-CRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-CRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded mouse retina. Anti- CRX antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of CRX in tumor tissue.

    • Expression of CRX in human tissue.

    • Expression of CRX in mouse & rat tissue.

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