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产品中心 抗体 病理/IHC抗体

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FSHR Recombinant Rabbit mAb (SDT-2269-36)

Follicle-stimulating hormone receptor,FSH-R,Follitropin receptor,LGR1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2402
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    FSHR

  • 分子别名

    Follicle-stimulating hormone receptor; FSH-R; Follitropin receptor; LGR1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    P23945

  • 克隆号

    SDT-2269-36

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    SK-OV-3, OVCAR-3, DU 145, PC-3, mouse kidney, rat testis

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Hu
    IF 1:100 Ms
背景介绍

  • The follicle-stimulating hormone receptor (FSHR) is a 695-amino-acid, seven-transmembrane G-protein-coupled receptor expressed primarily on granulosa and Sertoli cells; upon binding FSH it activates the Gs/adenylyl cyclase/cAMP/PKA cascade to regulate steroidogenesis, gametogenesis, and reproductive tract differentiation, with naturally occurring activating or inactivating mutations causing disorders such as ovarian hyperstimulation syndrome or hypergonadotropic hypogonadism, and its extracellular domain being the target of modern infertility therapies and male contraceptive development.

  • 免疫印迹

    • WB result of FSHR Recombinant Rabbit mAb
      Primary antibody: FSHR Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: SK-OV-3 whole cell lysate 20 µg
      Lane 2: OVCAR-3 whole cell lysate 20 µg
      Lane 3: DU 145 whole cell lysate 20 µg
      Lane 4: PC-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 78 kDa
      Observed MW: 70 kDa

    • WB result of FSHR Recombinant Rabbit mAb
      Primary antibody: FSHR Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 78 kDa
      Observed MW: 70 kDa

    • WB result of FSHR Recombinant Rabbit mAb
      Primary antibody: FSHR Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 78 kDa
      Observed MW: 70 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-FSHR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-FSHR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FSHR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-FSHR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse ovary. Anti-FSHR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat ovary. Anti-FSHR antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in SK-OV-3 cells. Anti- FSHR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded mouse ovary. Anti- FSHR antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of FSHR in tumor tissue.

    • Expression of FSHR in human tissue.

    • Expression of FSHR in mouse & rat tissue.

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