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宿主来源
Rabbit抗原名称
GRM5分子别名
Metabotropic glutamate receptor 5; mGluR5; GPRC1E免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P41594克隆号
SDT-2419-37抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse brain, mouse hippocampus, rat brain, rat hippocampus纯化方式
Protein A浓度
1mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
GRM5 encodes mGluR5, a Gq-coupled G-protein–coupled receptor selectively expressed at neuronal postsynaptic sites where it senses extracellular glutamate and triggers a phosphatidylinositol-calcium second-messenger cascade that modulates synaptic plasticity and network excitability, thereby influencing cognition, addiction, anxiety and other behaviors, while aberrant GRM5 signaling has been implicated in disorders ranging from schizophrenia and autism spectrum disorder to cancer metastasis and neurodegeneration.
免疫印迹
WB result of GRM5 Recombinant Rabbit mAb
Primary antibody: GRM5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse hippocampus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 132 kDa
Observed MW: 140-300 kDaWB result of GRM5 Recombinant Rabbit mAb
Primary antibody: GRM5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat hippocampus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 132 kDa
Observed MW: 140-300 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GRM5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-GRM5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-GRM5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-GRM5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-GRM5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of GRM5 in tumor tissue.
Expression of GRM5 in human tissue.
Expression of GRM5 in mouse & rat tissue.
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