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宿主来源
Rabbit抗原名称
MUC5B分子别名
Mucin-5B; MUC-5B; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B; tracheobronchial; Sublingual gland mucin; MUC5免疫原
Synthetic Peptide细胞定位
SecretedAccession
Q9HC84克隆号
SDT-2351-40抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
1mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4°C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu
MUC5B, encoded on chromosome 11p15.5, is a 5,700-amino-acid gel-forming mucin produced chiefly by mucosal epithelial cells in the respiratory tract, gastrointestinal tract, cervix, and salivary glands; it polymerizes via N-terminal von Willebrand factor-like D1-D2-D′-D3 domains that disulfide-link into massive, highly glycosylated (O-linked) linear chains whose extensive carbohydrate content (up to 80 % of mass) endows viscoelastic, lubricating, and antimicrobial barrier properties essential for mucociliary clearance and host defense.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MUC5B in tumor tissue.
Expression of MUC5B in human tissue.
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