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宿主来源
Rabbit抗原名称
MUC5B分子别名
Mucin-5B; MUC-5B; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B; tracheobronchial; Sublingual gland mucin; MUC5免疫原
Synthetic Peptide细胞定位
SecretedAccession
Q9HC84克隆号
SDT-2351-40抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu IF 1:500 Hu
MUC5B, encoded on chromosome 11p15.5, is a 5,700-amino-acid gel-forming mucin produced chiefly by mucosal epithelial cells in the respiratory tract, gastrointestinal tract, cervix, and salivary glands; it polymerizes via N-terminal von Willebrand factor-like D1-D2-D′-D3 domains that disulfide-link into massive, highly glycosylated (O-linked) linear chains whose extensive carbohydrate content (up to 80 % of mass) endows viscoelastic, lubricating, and antimicrobial barrier properties essential for mucociliary clearance and host defense.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human colon. Anti- MUC5B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded human colon. Anti- MUC5B antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of MUC5B in tumor tissue.
Expression of MUC5B in human tissue.
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