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Rabbit抗原名称
TFEB分子别名
Transcription factor EB; Class E basic helix-loop-helix protein 35 (bHLHe35); BHLHE35免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, LysosomeAccession
P19484克隆号
SDT-2411-7抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Raji, Ramos, RAW264.7, rat heart纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu, Ms
TFEB protein, which stands for Transcription Factor EB, is a crucial regulator of lysosomal biogenesis and autophagy. It is a member of the MiT family of transcription factors. When TFEB is activated, it translocates to the nucleus and binds to the promoters of various genes related to lysosomal and autophagy functions, thereby promoting the expression of these genes. This process helps cells to enhance their ability to degrade and recycle cellular components, which is essential for maintaining cellular homeostasis, especially under conditions of cellular stress such as nutrient deprivation or the presence of damaged organelles. Abnormal regulation of TFEB has been implicated in several diseases, including neurodegenerative diseases and lysosomal storage disorders, making it a potential therapeutic target.
免疫印迹
WB result of TFEB Recombinant Rabbit mAb
Primary antibody: TFEB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDaWB result of TFEB Recombinant Rabbit mAb
Primary antibody: TFEB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDaWB result of TFEB Recombinant Rabbit mAb
Primary antibody: TFEB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TFEB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-TFEB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-TFEB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TFEB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Raji cells. Anti- TFEB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Raw264.7 cells. Anti-TFEB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TFEB in tumor tissue.
Expression of TFEB in human tissue.
Expression of TFEB in mouse & rat tissue.
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