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Rabbit抗原名称
PAD4分子别名
Protein-arginine deiminase type-4; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV; Pad4; Pdi4; Padi4免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q9Z183克隆号
SDT-2416-3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IHC-P 1:500-1:1000 Ms, Rt
Protein-arginine deiminase type-4 (PAD4) is a human enzyme encoded by the PADI4 gene, located on the short arm of chromosome 1. PAD4 is a calcium-dependent enzyme that catalyzes the conversion of arginine residues to citrulline in proteins, a process known as citrullination. It is primarily expressed in white blood cells such as granulocytes and monocytes, and can also be found in the nucleus. PAD4 plays a crucial role in the formation of neutrophil extracellular traps (NETs), which are involved in the immune response against pathogens. However, excessive PAD4 activity and NET formation have been implicated in various diseases, including rheumatoid arthritis, multiple sclerosis, cancer, and cardiovascular diseases. PAD4 also influences gene expression by deimininating histones, and its dysregulation can impact cellular processes like apoptosis and differentiation. Due to its involvement in multiple disease pathways, PAD4 has become a promising target for therapeutic interventions.
免疫印迹
WB result of PAD4 Recombinant Rabbit mAb
Primary antibody: PAD4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-PAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-PAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-PAD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-PAD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-PAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PAD4 in mouse & rat tissue.
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