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宿主来源
Mouse细胞定位
Cytoplasm克隆号
SDT-02921抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000-1:2000 Hu
The Human Cytoplasmic Marker antibody is a mouse-derived monoclonal antibody that specifically targets a human cytoplasmic antigen, particularly in neural cells, without cross-reacting with the cytoplasm of mouse, rat, or monkey cells. This makes it highly valuable for tracking the survival, migration, and differentiation of human cells in xenotransplantation studies. STEM121 is commonly used in immunohistochemistry and immunofluorescence to label human cells transplanted into model animals (e.g., mice, rats), ensuring experimental accuracy and reliability. In earlier literature, it was also referred to as SC121 and has been widely applied in neural stem cell transplantation research.
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Human Cytoplasmic Marker antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Human Cytoplasmic Marker antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Human Cytoplasmic Marker antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Human Cytoplasmic Marker antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Human Cytoplasmic Marker antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Human Cytoplasmic Marker in tumor tissue.
Expression of Human Cytoplasmic Marker in mouse & rat tissue.
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