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宿主来源
Rabbit抗原名称
NUT分子别名
NUT family member 1; Nuclear protein in testis; C15orf55; NUTM1细胞定位
Cytoplasm, NucleusAccession
Q86Y26克隆号
SDT-3062抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Rt IHC-P 1:250 Hu, Rt IF 1:500 Hu, Rt
The NUT protein, which stands for nuclear protein in testis, is a transcription factor that plays a crucial role in embryonic development. It is typically found in the testes and is involved in the regulation of gene expression. However, when it is abnormally expressed or translocated in other tissues, it can lead to the development of certain types of cancers, particularly NUT midline carcinoma (NMC), a rare but aggressive malignancy. The presence of the NUT protein in cancer cells is often associated with poor prognosis, and it has become an important target for research in cancer diagnostics and therapeutics.
免疫印迹
WB result of NUT Recombinant Rabbit mAb
Primary antibody: NUT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 120 kDa
Observed MW: 150 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-NUT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human midline carcinoma. Anti-NUT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-NUT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded rat testis. Anti-NUT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of NUT in tumor tissue.
Expression of NUT in human tissue.
Expression of NUT in mouse & rat tissue.
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