PIK3CD Recombinant Rabbit mAb (SDT-1978-148)
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform,PI3-kinase subunit delta,PI3K-delta,PI3Kdelta,PtdIns-3-kinase subunit delta,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta (PtdIns-3-kinase subunit p110-delta,p110delta)
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宿主来源
Rabbit抗原名称
PIK3CD分子别名
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta (PtdIns-3-kinase subunit p110-delta; p110delta)免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
O00329克隆号
SDT-1978-148抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
Jurkat, K562, HT-1080, moues spleen, mouse brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:250 Hu, Ms ICC 1:500 Hu
PIK3CD is a gene that encodes a protein which is an integral part of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. This pathway is crucial for various cellular functions including cell growth, proliferation, survival, and metabolism. The PIK3CD protein specifically refers to the delta isoform of the catalytic subunit of PI3K. It is predominantly expressed in immune cells and plays a significant role in the regulation of immune responses. Abnormal activation or mutations in the PIK3CD gene can lead to a range of diseases including certain types of leukemia and other immune-related disorders. Understanding its function and regulation is important for developing targeted therapies in cancer and immune system disorders.
免疫印迹
WB result of PIK3CD Recombinant Rabbit mAb
Primary antibody: PIK3CD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDaWB result of PIK3CD Recombinant Rabbit mAb
Primary antibody: PIK3CD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-PIK3CD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- PIK3CD antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PIK3CD in tumor tissue.
Expression of PIK3CD in human tissue.
Expression of PIK3CD in mouse & rat tissue.
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