VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb (SDT-1519-144)
Vascular endothelial growth factor receptor 2,VEGFR-2,Fetal liver kinase 1 (FLK-1),Kinase insert domain receptor (KDR),Protein-tyrosine kinase receptor flk-1,CD309,FLK1,VEGFR2,KDR
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宿主来源
Rabbit抗原名称
VEGF Receptor 2分子别名
Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1 (FLK-1); Kinase insert domain receptor (KDR); Protein-tyrosine kinase receptor flk-1; CD309; FLK1; VEGFR2; KDR免疫原
Recombinant Protein细胞定位
Cell membrane, Endoplasmic reticulum, Cell junctionAccession
P35968克隆号
SDT-1519-144抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HUVEC纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu, Ms, Rt
VEGF Receptor 2 (VEGFR-2), also known as KDR in humans and Flk-1 in mice, is a crucial receptor tyrosine kinase that plays a central role in angiogenesis, the formation of new blood vessels. This receptor has high affinity for VEGF-A and mediates most of the endothelial growth, migration, and survival signals induced by VEGF-A. Structurally, VEGFR-2 is a glycosylated transmembrane protein with a molecular weight of about 230 kDa, consisting of an extracellular domain (ECD) for ligand binding, a transmembrane domain (TMD), a juxtamembrane domain (JMD), a tyrosine kinase domain (TKD), and a C-terminal domain (CTD) for signaling. Upon binding to VEGF-A, VEGFR-2 undergoes dimerization and autophosphorylation, activating downstream signaling pathways such as the PLCγ-PKC-MAPK pathway, which promotes endothelial cell proliferation, migration, and vascular permeability. VEGFR-2 is widely expressed in vascular endothelial cells and is also found in some tumor cells, making it a key target for both pro-angiogenic therapies in conditions like ischemic diseases and anti-angiogenic therapies in cancer.
免疫印迹
WB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 250-280 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-VEGF Receptor 2/FLK-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-VEGF Receptor 2/FLK-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-VEGF Receptor 2/FLK-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-VEGF Receptor 2/FLK-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-VEGF Receptor 2/FLK-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-VEGF Receptor 2/FLK-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VEGF Receptor 2/FLK-1 in tumor tissue.
Expression of VEGF Receptor 2/FLK-1 in human tissue.
Expression of VEGF Receptor 2/FLK-1 in mouse & rat tissue.
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