Myosin Light Chain 2/MLC-2V Rabbit Polyclonal Antibody
Myosin regulatory light chain 2,ventricular/cardiac muscle isoform,MLC-2,Cardiac myosin light chain 2,Myosin light chain 2,slow skeletal/ventricular muscle isoform (MLC-2s/v),Ventricular myosin light chain 2,MLC2,MYL2
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宿主来源
Rabbit抗原名称
Myosin Light Chain 2/MLC-2V分子别名
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; Cardiac myosin light chain 2; Myosin light chain 2; slow skeletal/ventricular muscle isoform (MLC-2s/v); Ventricular myosin light chain 2; MLC2; MYL2免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P10916抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Mouse heart muscle, rat heart muscle, human heart muscle预测反应种属
(反应种属缩写表)Bv, Rb纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt IF 1:500 Ms, Rt
Myosin Light Chain 2 (MLC-2V) is a critical sarcomeric protein predominantly found in the ventricular muscle of the heart. It plays a significant role in regulating cardiac muscle contraction through its phosphorylation, which affects myosin cycling kinetics, torsion, and overall function. MLC-2V is part of the myosin complex, a hexameric protein that includes myosin heavy chains and light chains. Its specific isoform in the ventricle, MLC-2V, is essential for maintaining proper cardiac function. Mutations in the MLC-2V gene are associated with severe conditions such as hypertrophic cardiomyopathy and infant-onset myopathy. Additionally, recent studies have highlighted its involvement in the structural integrity of both cardiac and slow-twitch skeletal muscles, further emphasizing its importance in muscle physiology.
免疫印迹
WB result of Myosin Light Chain 2/MLC-2V Rabbit pAb
Primary antibody: Myosin Light Chain 2/MLC-2V Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 19 kDaWB result of Myosin Light Chain 2/MLC-2V Rabbit pAb
Primary antibody: Myosin Light Chain 2/MLC-2V Rabbit pAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 19 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Myosin Light Chain 2/ MLC-2V in tumor tissue.
Expression of Myosin Light Chain 2/ MLC-2V in human tissue.
Expression of Myosin Light Chain 2/ MLC-2V in mouse and rat tissue.
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