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MAFA Recombinant Rabbit mAb (SDT-2040-6)

Transcription factor MafA,Pancreatic beta-cell-specific transcriptional activator,RIPE3b1 factor,V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2367
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    MAFA

  • 分子别名

    Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1 factor; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q8NHW3

  • 克隆号

    SDT-2040-6

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Beta-TC-6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
    IHC-P 1:200 Hu, Ms, Rt
背景介绍

  • The MAFA protein (Musculoaponeurotic Fibrosarcoma Oncogene Homolog A) is a member of the basic leucine zipper (bZIP) transcription factor family, primarily expressed in pancreatic β-cells, the retina, and lymphocytes. It plays a critical role in regulating the transcription of the insulin gene (INS) by binding to the C1 element in the insulin gene promoter region, thereby promoting insulin synthesis and secretion to maintain glucose homeostasis. The expression and activity of MAFA are regulated by various factors, including glucose concentration, cytokines, and oxidative stress. In diabetes research, dysfunction of MAFA is closely associated with β-cell dysfunction and impaired insulin secretion, making it a potential therapeutic target. Additionally, MAFA also plays important roles in retinal development and immune regulation.

  • 免疫印迹

    • WB result of MAFA Recombinant Rabbit mAb
      Primary antibody: MAFA Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: C2C12 whole cell lysate 20 µg
      Lane 3: Beta-TC-6 whole cell lysate 20 µg
      Negative control: NIH/3T3 whole cell lysate; C2C12 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 40 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human pancreas. Anti-MAFA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-MAFA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-MAFA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat pancreas. Anti-MAFA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of MAFA in tumor tissue.

    • Expression of MAFA in human tissue.

    • Expression of MAFA in mouse & rat tissue.

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