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宿主来源
Rabbit抗原名称
PAX6分子别名
Paired box protein Pax-6; Aniridia type II protein; Oculorhombin; AN2免疫原
Synthetic Peptide细胞定位
NucleusAccession
P26367克隆号
SDT-1258-145抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
293T, Neuro-2a纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt
PAX6, also known as Paired Box 6, is a highly conserved transcription factor that plays a crucial role in the development of multiple organs and tissues, particularly the eyes, brain, and pancreas. It is involved in the regulation of gene expression during embryonic development and is essential for the formation of the eyes, central nervous system, and endocrine pancreas. Mutations in the PAX6 gene can lead to a variety of conditions, including aniridia (absence of the iris), microphthalmia (small eyes), and coloboma (gaps in eye structures). Additionally, PAX6 is implicated in the development of the olfactory bulb and the regulation of neuronal migration and cell proliferation. Its importance is further highlighted by its role in maintaining the normal function of certain cells after birth.
免疫印迹
WB result of PAX6 Recombinant Rabbit mAb
Primary antibody: PAX6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDaWB result of PAX6 Recombinant Rabbit mAb
Primary antibody: PAX6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human retina. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse retina. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat retina. Anti-PAX6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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