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SOX17 Recombinant Rabbit mAb (SDT-1803-48)

Transcription factor SOX-17

货号： S0B2361

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SOX17

  • 分子别名

    Transcription factor SOX-17

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Nucleus

  • Accession

    Q9H6I2

  • 克隆号

    SDT-1803-48

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu

  • 阳性样本

    SK-OV-3, OVCAR-3

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:1000 Hu
ICC 1:500 1:500
背景介绍

  • SOX17 protein, a member of the SOX (SRY-related HMG-box) family of transcription factors, is crucial for the regulation of embryonic development and cell fate determination. It plays a key role in endoderm development, cardiovascular system formation, maintenance of fetal hematopoietic stem cells, and postnatal angiogenesis. SOX17 also functions as a transcriptional regulator that binds to specific DNA sequences and modulates transcriptional regulation via WNT3A, inhibiting Wnt signaling and promoting the degradation of activated CTNNB1. Furthermore, it has been implicated in cancer, particularly as a mutated cancer driver gene in endometrial carcinoma, with missense mutations potentially converting SOX17 into a pluripotency-inducing factor and altering its DNA binding preferences and gene expression programs. SOX17 is also considered to act as a tumor suppressor through the attenuation of WNT/β-catenin signaling in various cancer types.

  • 免疫印迹

    • WB result of SOX17 Recombinant Rabbit mAb
      Primary antibody: SOX17 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: SK-OV-3 whole cell lysate 20 µg
      Lane 2: OVCAR-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 44 kDa
      Observed MW: 50 kDa

  • 免疫沉淀

    • SOX17 Recombinant Rabbit mAb at 1/50 dilution (1µg) immunoprecipitating SOX17 in 0.4 mg SK-OV-3 whole cell lysate.
      Western blot was performed on the immunoprecipitate using SOX17 Recombinant Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: SK-OV-3 whole cell lysate 40 µg (Input)
      Lane 2: SOX17 Recombinant Rabbit mAb IP in SK-OV-3 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in SK-OV-3 whole cell lysate
      Predicted MW: 44 kDa
      Observed MW: 50 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human ovary. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human seminoma. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in SK-OV-3 cells. Anti- SOX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of SOX17 in tumor tissue.

    • Expression of SOX17 in human tissue.

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