p63 Recombinant Rabbit mAb (SDT-054-137)
p40,TP63,KET,P63,P73H,P73L,TP73L,Tumor protein 63,p63,Chronic ulcerative stomatitis protein,CUSP,Keratinocyte transcription factor KET,Transformation-related protein 63,Tumor protein p73-like,p73L,p51,Chronic ulcerative stomatitis protein (CUSP),Transformation-related protein 63 (TP63),Tumor protein p73-like (p73L)
货号： S0B2360
- 价格： ￥600
- 规格：
- 数量：
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别名
p40,TP63,KET,P63,P73H,P73L,TP73L,Tumor protein 63,p63,Chronic ulcerative stomatitis protein,CUSP,Keratinocyte transcription factor KET,Transformation-related protein 63,Tumor protein p73-like,p73L,p51,Chronic ulcerative stomatitis protein (CUSP),Transformation-related protein 63 (TP63),Tumor protein p73-like (p73L)宿主来源
Rabbit抗原名称
p63免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9H3D4克隆号
SDT-054-137抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB, IP反应种属 ?
Hu阳性样本
HaCaT, A431纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu |
Tumor protein p63, typically referred to as p63, also known as transformation-related protein 63 is a protein that in humans is encoded by the TP63 (also known as the p63) gene. Transcription factor p63 is a key regulator of epidermal keratinocyte proliferation and differentiation. Tumor protein p63 is a member of the p53 family of transcription factors. p63 regulates the activity of a multitude of genes involved in growth and development of the ectoderm and derived structures and tissues, such as basal layer keratins and cell cycle control genes. Accordingly, p63 expression is found in basal cell layers of various organs, squamous epithelial cells of many organs and urothelium. p63 (TAp63) is closely related to p40 (ΔNp63) as both proteins represent isoforms of the p63 gene with distinct molecular functions. While “full length” p63 (TAp63) activates p53 target genes such as p21 or BAX, the shorter transcript p40 (ΔNp63) inhibits activation of p53 and “full length” p63. P63 is also helpful in distinguishing poorly differentiated squamous cell carcinoma from small cell carcinoma or adenocarcinoma. P63 should be strongly stained in poorly differentiated squamous cell, but negative in small cell or adenocarcinoma.
免疫印迹
WB result of p63 Recombinant Rabbit mAb
Primary antibody: p63 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HaCaT whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 76 kDa
Observed MW: 76 kDa
免疫沉淀
p63 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p63 in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using p63 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: A431 whole cell lysate 20 µg (Input)
Lane 2: p63 Recombinant Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 76kDa
Observed MW: 76kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-p63 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-p63 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p63 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-p63 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HaCaT cells. Anti-p63 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of p63 in tumor tissue.
Expression of p63 in human tissue.
Expression of p63 in lung cancer TMA.
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