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S-RMab® B7-H4 Recombinant Rabbit mAb,PBS Only (SDT-1408-111) STAR

V-set domain-containing T-cell activation inhibitor 1,B7 homolog 4,B7h.5,Protein B7S1,T-cell costimulatory molecule B7x,VTCN1

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货号 S0B2356P
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    B7-H4

  • 分子别名

    V-set domain-containing T-cell activation inhibitor 1; B7 homolog 4; B7h.5; Protein B7S1; T-cell costimulatory molecule B7x; VTCN1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    Q7Z7D3

  • 克隆号

    SDT-1408-111

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 阳性样本

    SK-BR-3, T-47D

  • 纯化方式

    Protein A

  • 浓度

    1 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
    IHC-P 1:150 Hu
背景介绍

  • B7-H4 is a transmembrane protein that binds an unknown receptor on activated T cells resulting in inhibition of T-cell effector function via cell cycle arrest, decreased proliferation, and reduced IL-2 production. B7-H4 is up-regulated on the surface of cancer cells and immunosuppressive tumor-associated macrophages (TAMs) in a variety of human cancers. Notably, B7-H4 expression levels inversely correlate with patient survival in ovarian cancer, cholangiocarcinoma, breast cancer and endometrial cancer, making B7-H4 an attractive candidate for therapeutic intervention.

  • 免疫印迹

    • WB result of S-RMab® B7-H4 Recombinant Rabbit mAb
      Primary antibody: S-RMab® B7-H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: SK-BR-3 whole cell lysate 20 µg
      Lane 3: T-47D whole cell lysate 20 µg
      Negative control: HeLa whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 25, 65 kDa

    • WB result of S-RMab® B7-H4 Recombinant Rabbit mAb
      Primary antibody: S-RMab® B7-H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: recombinant human B7-H4 protein 20ng (UA010121)
      Lane 2: recombinant human B7-H1 protein 20ng (S0A6006)
      Lane 3: recombinant human B7-H2 protein 20ng (UA010058)
      Lane 4: recombinant human B7-H3 protein 20ng (UA010010)
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 38~60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human breast cancer (case 1). Anti- B7-H4 antibody was used at 1/150 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer (case 2). Anti- B7-H4 antibody was used at 1/150 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer (case 3). Anti- B7-H4 antibody was used at 1/150 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer (case 1). Anti- B7-H4 antibody was used at 1/150 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer (case 2). Anti- B7-H4 antibody was used at 1/150 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human breast. Anti- B7-H4 antibody was used at 1/150 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of B7-H4 in tumor tissue

    • Expression of B7-H4 in human tissue

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