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Rabbit抗原名称
Claudin 1分子别名
Senescence-associated epithelial membrane protein; CLDN1; CLD1, SEMP1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
O95832克隆号
SDT-1289-55抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IF反应种属 ?
Hu阳性样本
MCF7, A431, HepG2, mouse skin, rat kidney预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
| IF | 1:200 | Hu |
Claudin 1 is a 23 kDa multi-pass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. It mainly expresses the peripheral neuroma (partial type of negative), derivative skin fibrous sarcoma, fibroma and deity fibroma.
免疫印迹
WB result of Claudin 1 Recombinant Rabbit mAb
Primary antibody: Claudin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDaWB result of Claudin 1 Recombinant Rabbit mAb
Primary antibody: Claudin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDaWB result of Claudin 1 Recombinant Rabbit mAb
Primary antibody: Claudin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human synovialsarcoma. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human dermatofibrosarcoma protuberans. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin 1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) (S0B4051) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human cervical cancer. Anti-Claudin 1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) (S0B4051) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human liver cancer. Anti-Claudin 1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) (S0B4051) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Claudin 1 in tumor tissue.
Expression of Claudin 1 in human tissue.
Expression of Claudin 1 in mouse & rat tissue.
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