大包装询价 产品介绍 引用文献(0) 评论(0)
宿主来源
Rabbit抗原名称
MUC17分子别名
Mucin-17; Small intestinal mucin-3 (MUC-3)免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q685J3克隆号
SDT-1415-49抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, IF反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:500 |
| IF | 1:500 |
Mucin-17 (MUC17) is a membrane-tethered mucin and a member of the mucin family. Mucin family members play crucial roles in protecting epithelial cells, providing lubrication, maintaining epithelial integrity, aiding in cell adhesion, and offering protection against pathogens, toxins, and foreign particles. MUC17's expression changes have been observed in various pathological conditions, especially in cancer and inflammatory bowel diseases. The biosynthesis of MUC17 occurs in the endoplasmic reticulum of goblet cells and may undergo N-glycosylation either before or after dimerization. In tumor tissues, the abnormal expression of MUC17 is correlated with tumor invasion, metastasis, and prognosis. In recent years, MUC17 has been recognized as an important biomarker for certain cancers and a promising therapeutic target. Particularly, the role of MUC17 in gastric cancer (GC) has attracted researchers' attention. According to one study, MUC17 is highly expressed in gastric cancer tissues and is associated with a favorable prognosis for patients.
免疫组化
IHC shows positive staining in paraffin-embedded human small intestine. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human small intestine. Anti-MUC17 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
引用文献(0)
评论(0)