NaPi2b/SLC34A2 Recombinant Rabbit mAb (SDT-R229)
NPT2B,Sodium-dependent phosphate transport protein 2B,Sodium-phosphate transport protein 2B,Na(+)-dependent phosphate cotransporter 2B,NaPi3b,Sodium/phosphate cotransporter 2B,Na(+)/Pi cotransporter 2B,NaPi-2b,Solute carrier family 34 member 2,SLC34A2,NaPi2b/SLC34A2,Sodium/phosphate cotransporter 2B (Na(+)/Pi cotransporter 2B,NaPi-2b)
货号： S0B2327
- 价格： ￥600
- 规格：
- 数量：
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别名
NPT2B,Sodium-dependent phosphate transport protein 2B,Sodium-phosphate transport protein 2B,Na(+)-dependent phosphate cotransporter 2B,NaPi3b,Sodium/phosphate cotransporter 2B,Na(+)/Pi cotransporter 2B,NaPi-2b,Solute carrier family 34 member 2,SLC34A2,NaPi2b/SLC34A2,Sodium/phosphate cotransporter 2B (Na(+)/Pi cotransporter 2B,NaPi-2b)宿主来源
Rabbit抗原名称
NaPi2b/SLC34A2细胞定位
Apical cell membraneAccession
O95436克隆号
SDT-R229抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
| ICC | 1:500 |
Sodium-dependent phosphate transport protein 2B (NaPi2b) is a protein that in humans is encoded by the SLC34A2 gene. NaPi2b is a cell surface sodiumdependent phosphate transporter that regulates phosphate homeostasis under normal physiological conditions and is a lineage marker that is expressed in select cancers, including ovarian, lung, thyroid, and breast cancers, with limited expression in normal tissues. Based on its increased expression in ovarian tumors, NaPi2b is a promising candidate to be studied as a biomarker for treatment and patient selection in ovarian cancer. In preclinical studies, the use of antibodies against NaPi2b showed that this protein can be exploited for tumor mapping and therapeutic targeting.
免疫印迹
WB result of NaPi2b/SLC34A2 Recombinant Rabbit mAb
Primary antibody: NaPi2b/SLC34A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HepG2 whole cell lysate 20 µg
Lane 2: unboiled OVCAR-3 whole cell lysate 20 µg
Negative control: unboiled HepG2 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90~130 kDa
Observed MW: 90~130 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human lung. Anti- NaPi2b/SLC34A2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast. Anti- NaPi2b/SLC34A2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti- NaPi2b/SLC34A2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- NaPi2b/SLC34A2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- NaPi2b/SLC34A2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti- NaPi2b/SLC34A2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in OVCAR-3 cells (top panel) and negative staining in HepG2 cells (below panel). Anti-NaPi2b/SLC34A2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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