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EpCAM/CD326 Recombinant Rabbit mAb (S-1009-76)

Epithelial cell adhesion molecule,Ep-CAM,Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein (EGP),Epithelial glycoprotein 314 (EGP314,hEGP314),KS 1/4 antigen,KSA,Major gastrointestinal tumor-associated protein GA733-2,Tumor-associated calcium signal transducer 1,EPCAM,GA733-2,M1S2,M4S1,MIC18,TACSTD1,TROP1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B2316

规格

25μl100μl500μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein (EGP), Epithelial glycoprotein 314 (EGP314; hEGP314), KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
免疫原

Recombinant Protein
细胞定位

Cell junction
Accession

P16422
克隆号

S-1009-76
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
ICC 1:500
FCM 1:5000

应用	稀释度
WB	1:1000
IP	1:50
ICC	1:500
FCM	1:5000

背景介绍

Epithelial cell adhesion molecule (EpCAM), also known as CD326 among other names, is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

免疫印迹
- WB result of CD326 Rabbit mAb
  Primary antibody: CD326 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MB-MDA-231 whole cell lysate 20 µg
  Lane 3: HT-29 whole cell lysate 20 µg
  Lane 4: T-47D whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate; MB-MDA-231 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 37 kDa
流式分析
- Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / HT29 (Human colorectal adenocarcinoma epithelial cell, Right) cells labelling CD326 antibody at 1/5000 dilution (0.01 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti- Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: Jurkat
免疫细胞化学
- ICC shows positive staining in HT-29 cells (top panel) and negative staining in MDA-MB-231 cells (below panel). Anti-CD326 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).