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CD19 Mouse mAb (S-1039-46)

B-lymphocyte antigen CD19,LE-CD19,B-lymphocyte surface antigen B4,Differentiation antigen CD19,T-cell surface antigen Leu-12

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B2311

规格

25μl100μl500μl1ml

数量

产品介绍

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产品规格

宿主来源

Mouse
抗原名称

CD19
分子别名

B-lymphocyte antigen CD19, LE-CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P15391
克隆号

S-1039-46
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

FCM

ICC ?

WB

IF ?
稀释度

应用稀释度

WB 1:1000
IHC-P 1:500-1:1000
ICC 1:500
FCM 1:2000
IF 1:500

应用	稀释度
WB	1:1000
IHC-P	1:500-1:1000
ICC	1:500
FCM	1:2000
IF	1:500

背景介绍

The CD19 antigen plays an important role in clinical oncology. In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry [PMID: 8528044].

免疫印迹
- WB result of CD19 Mouse mAb
  Primary antibody: CD19 Mouse mAb at 1/1000 dilution
  Lane 1: untreated Raji whole cell lysate 20 µg
  Lane 2: Raji treated with PNGase F whole cell lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 61 kDa
  Observed MW: 95 kDa
流式分析
- Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD19 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
免疫组化
- IHC shows positive staining in paraffin-embedded human spleen. Anti-CD19 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD19 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD19 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD19 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Raji cells (top panel) and negative staining in Jurkat cells (below panel). Anti-CD19 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
- IF shows positive staining in paraffin-embedded human tonsil. Anti-CD19 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.